EC Number   |
Protein Variants |
Reference |
|---|
   5.4.99.11 | D140E |
almost complete loss of catalytic activity |
-, 679557 |
| 5.4.99.11 | D140G |
almost complete loss of catalytic activity |
-, 679557 |
| 5.4.99.11 | D140N |
almost complete loss of catalytic activity |
-, 679557 |
| 5.4.99.11 | D200A |
enzyme mutant structure analysis |
-, 746878 |
| 5.4.99.11 | D241A |
site-directed mutagenesis, glucose binding structure in comparison to the wild-type enzyme by crystal structure analysis |
728625 |
| 5.4.99.11 | D327N |
turnover number for sucrose: 1.4fold decrease, KM-value for sucrose: 1.65fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 43.7% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 41.0% increase |
651318 |
| 5.4.99.11 | D327R |
turnover number for sucrose: 2.45fold decrease, KM-value for sucrose: 2.2fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 26.5% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 17.7% increase |
651318 |
| 5.4.99.11 | D329A |
42% of wild-type activity, no change in major products |
-, 679557 |
| 5.4.99.11 | D329N |
turnover number for sucrose: 1.17fold decrease, KM-value for sucrose: 2.9fold increase, alpha-D-glucopyranosyl-1,6-D-fructofuranose content: 52.4% decrease, alpha-D-glucopyranosyl-1,1-D-fructofuranose content: 54.7% increase |
651318 |
| 5.4.99.11 | D442N |
the mutant favors the transfer reaction with an isomer preference for isomaltulose |
-, 701813 |
