EC Number   |
Protein Variants |
Reference |
|---|
    5.4.4.2 | A303T |
the kcat/Km (chorismate) for the A303T mutant is 130fold lower than that for wild type enzyme |
705184 |
| 5.4.4.2 | A303T/F327Y |
the mutant shows no detectable activity |
705184 |
| 5.4.4.2 | A303T/F327Y/F359Q |
the mutant shows no detectable activity |
705184 |
| 5.4.4.2 | A303T/F327Y/F359Q/I346L |
the mutant shows no detectable activity |
705184 |
| 5.4.4.2 | A303T/F327Y/I346L |
the mutant shows no detectable activity |
705184 |
| 5.4.4.2 | A303T/F359Q |
the mutant shows no detectable activity |
705184 |
| 5.4.4.2 | A344T |
no catalytic activity |
678320 |
| 5.4.4.2 | E240Q |
no catalytic activity |
678320 |
| 5.4.4.2 | E269A |
site-directed mutagenesis, the residue acts as general acid, inactive mutant |
726817 |
| 5.4.4.2 | E313A |
site-directed mutagenesis, the residue chelates Mg2+ and acts as general base, mutant with highly reduced activity compared to wild-type |
726817 |
