EC Number   |
Protein Variants |
Reference |
|---|
   5.1.99.3 | C184S |
the mutation leads to total loss of activity |
-, 715939 |
| 5.1.99.3 | C79S |
the mutation leads to total loss of activity |
-, 715939 |
| 5.1.99.3 | C79S/C184S |
the double mutation leads to total loss of activity |
-, 715939 |
| 5.1.99.3 | E78D |
mutant shows diminished catalytic activity affecting kcat and KM for both enantiomers |
-, 747111 |
| 5.1.99.3 | E78Q |
loss of catalytic activity |
-, 747111 |
| 5.1.99.3 | more |
gene insertion mutant, gene hpxA is not essential for growth on allantoin |
-, 692892 |
| 5.1.99.3 | more |
The Pseudomonas protein lacks a cysteine residue (C79 in the Klebsiella pneumoniae AllR sequence) that is essential for the two-base catalytic mechanism of KpAllR. The residue is substituted with glycine |
-, 747111 |
