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5.1.3.34
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generation of mgdE gene disruption mutants, disruption of the gene for the glucolipid epimerase (mgdE) in the monogalactosyldiacylglycerol (MGDG) synthase gene 1 (MGD1) background. The DELTAmgdE mutants retain about 70% of the chlorophyll content of the wild-type, no galactose moiety is detected in the deletion mutant by GC-MS analysis, confirming that monogalactosyldiacylglycerol comprise less than 0.4% of total membrane lipids. The mutants contain normal-looking thylakoid membranes. TLC analysis of lipids in the wild-type and DELTAmgdE cells of Synechocystis sp. PCC 6803 expressing GST-MGD1
735181
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