5.1.2.3 | G116F |
both the enoyl-CoA hydratase activity, EC 4.2.1.17 and the 3-hydroxyacyl-CoA epimerase activity, EC 5.1.2.3 are eliminated by the site-directed mutation alpha/Gly116 to Phe. This provides direct evidence that both enzymes are associated with a common active site on the amino-terminal domain of the multifunctional enzyme complex which exhibits the activities of EC 4.2.1.17, EC 2.3.1.16, EC 5.1.2.3, EC 1.1.1.35, and EC 5.3.3.3 |
2216 |