EC Number   |
Protein Variants |
Reference |
|---|
    5.1.1.7 | C217A |
mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen from L,L-3-fluoro-2,6-diaminopimelate, incapable of catalyzing HF elimination from D,L-3-fluoro-2,6-diaminopimelate |
651569 |
| 5.1.1.7 | C217S |
catalyzes epimerization of L,L-diaminopimelate at 2% of the activity of the wild-type enzyme,catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate |
651569 |
| 5.1.1.7 | C221A |
site-directed mutagenesis, nearly inactive mutant |
749346 |
| 5.1.1.7 | C226A |
complete loss of activity |
-, 692384 |
| 5.1.1.7 | C226S |
severely compromised catalytic efficiency |
-, 692384 |
| 5.1.1.7 | C73A |
mutant enzyme is inactive as epimerase, catalyzes elimination of HF via abstraction of the C-2 hydrogen. Mutant enzyme is able to rapidly catalyze elimination of the D,L-3-fluoro-2,6-diaminopimelate and is unable to catalyze elimination with the L,L-3-fluoro-2,6-diaminopimelate |
651569 |
| 5.1.1.7 | C73S |
epimerization of L,L-diaminopimelate at 3% of the activity of the wild-type enzyme, catalyzes HF elimination from L,L-3-fluoro-2,6-diaminopimelate and D,L-3-fluoro-2,6-diaminopimelate |
651569 |
| 5.1.1.7 | C73S/C217S |
in order to prevent C73 and C217 of DAP epimerase from oxidation to a disulfide prior to crystallization, DAP epimerase mutants C73S and C217S from Haemophilus influenzae are generated by site-directed mutagenesis |
671810 |
| 5.1.1.7 | C73S/C217S |
mutant enzyme is inactive as epimerase, slow elimination of HF from D,L-3-fluoro-2,6-diaminopimelate and L,L-3-fluoro-2,6-diaminopimelate |
651569 |
| 5.1.1.7 | C83A |
site-directed mutagenesis, nearly inactive mutant |
749346 |
