EC Number   |
Protein Variants |
Reference |
|---|
    4.99.1.2 | C159S |
site-directed mutagenesis, inactive mutant |
698997 |
| 4.99.1.2 | C160S |
50% inhibition by dithiothreitol in 20 mM cysteine |
650094 |
| 4.99.1.2 | C160S |
site-directed mutagenesis, mutant enzyme with moderately reduced activity |
698997 |
| 4.99.1.2 | C96S |
site-directed mutagenesis, inactive mutant |
698997 |
| 4.99.1.2 | D99N |
Cu(II) is bound to the active site cysteine residues |
747098 |
| 4.99.1.2 | D99S |
mutation mimicks the sequence of Bacillus megaterium MerB. Cu(II) is bound to the active site cysteine residues and is displaced following the addition of either an organomercurial substrate or an ionic mercury product |
747098 |
| 4.99.1.2 | more |
coexpression of enzyme and mercuric reductase in Arabidopsis thaliana leads to growth on 50fold higher methylmercury concentrations than wild type plants |
653291 |
| 4.99.1.2 | more |
engineering of enzyme as to be targeted for accumulation in the endoplasmic reticulum and for secretion to the cell wall rendering the expressing plants resistant to organic mercury |
653539 |
| 4.99.1.2 | more |
The organomercurial lyase gene of plasmid pMR26 (GenBank/DDBJ accession no. D83080) is amplified by PCR using two specific primers. The pBMK01 plasmid is transformed into Agrobacterium tumefaciens LBA4404 by electroporation, and its sequence is confirmed. The ppk/merT-transgenic tobacco (T3) is then transformed with this Agrobacterium tumefaciens, the tobacco callus shows more resistance to CH3Hg+ and accumulated significantly more mercury from CH3Hg+-containing medium than does the ppk/merT-transgenic and wild-type tobaccos |
701887 |
