EC Number   |
Protein Variants |
Reference |
|---|
   4.4.1.31 | C122L |
4% of wild-type activity |
731288 |
| 4.4.1.31 | C161L |
64% of wild-type activity |
731288 |
| 4.4.1.31 | C48Q |
78% of wild-type activity |
731288 |
| 4.4.1.31 | C91L |
181% of wild-type activity |
731288 |
| 4.4.1.31 | more |
when in subunit PecE the two motifs Y29YAAWWL and D263DLL are deleted, the holoenzyme loses its activity. It is also inactivated upon deletion of a central part, residues R111 to A122 |
731288 |
| 4.4.1.31 | more |
when in subunit PecF the 20 C-terminal and 56 N-terminal amino acids are truncated, the lyase-isomerase activity in combination with subunit PecE decreases to 12% and 15%, respectively, compared to the native enzyme. The catalytic efficiency (kcat/Km) decreases 16fold when the unique four histidine residues in PecF beginning at H53 are deleted. H121 and C122 of PecF are essential for the enzyme activity, they are part of a unique stretch extending from A104 to N125 which is absent in the beta-subunit of related but non-isomerizing lyases |
731288 |
