EC Number |
Protein Variants |
Reference |
---|
4.4.1.21 | C41A |
by site directed mutagenesis |
678153 |
4.4.1.21 | C82A |
inactive, mutation decreases autoinducer AI-2 production and biofilm formation |
749063 |
4.4.1.21 | C82S |
43fold decrease in activity, mutation decreases autoinducer AI-2 production and biofilm formation |
749063 |
4.4.1.21 | C83A |
mutant shows lower activity than the wild type enzyme |
692775 |
4.4.1.21 | C83D |
by site directed mutagenesis |
678153 |
4.4.1.21 | C84A |
no catalytic activity |
654703 |
4.4.1.21 | C84A |
site-directed mutagenesis, catalytically inactive mutant, the mutant contains a Co2+ ion, in the wild-type the substrate's cyclic ribosyl moiety is positioned adjacent to the Zn2+ ion, while in the mutant the noncyclic ribosyl is ligated to the Co2+ via its C2-O carbonyl oxygen |
730256 |
4.4.1.21 | C84D |
more than 220fold reduced activity |
654703 |
4.4.1.21 | C84D |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
730256 |
4.4.1.21 | C84S |
more than 220fold reduced activity |
654703 |