EC Number   |
Protein Variants |
Reference |
|---|
   4.4.1.14 | A46V |
modeling of the mutation, corresponding to A57V in Cucumis melo, which results in andromonoecious plants. The mutation changes the structure of the neighbouring active site residues only marginally. The mutation may cause an improper orientation of SAM in the active site |
715031 |
| 4.4.1.14 | E47D |
3.8% of wild-type ACC synthase activity |
650026 |
| 4.4.1.14 | E47Q |
0.9% of wild-type ACC synthase activity |
650026 |
| 4.4.1.14 | G289V |
naturally occuring mutation, no activity |
706316 |
| 4.4.1.14 | K273A |
no ACC synthase activity |
652810 |
| 4.4.1.14 | more |
- |
34580 |
| 4.4.1.14 | more |
a transient expression system in Arabidopsis protoplasts is used to determine if the interaction with 14-3-3 protein increases the half-life of ACS5. Coexpression of HA-14-3-3v increases the half-life of myc-tagged ACS5 protein in this system |
730603 |
| 4.4.1.14 | more |
deleltion of 46-52 amino acids from the COOH terminus results in an nine times higher affinity for S-adenosylmehtionine |
34586 |
| 4.4.1.14 | more |
deletion of 11 amino acids through Glu-23 from the N-terminus results in a substantial reduction of in vitro activity |
34579 |
| 4.4.1.14 | more |
deletion of residues 2-12 from the non-conserved N-terminus leads to slight increase in activity in vitro |
34579 |
