EC Number   |
Protein Variants |
Reference |
|---|
   4.3.1.1 | C141S/C274A |
elimination of sensitivity to inactivation |
649266 |
| 4.3.1.1 | H134A |
no loss of activity |
-, 651063 |
| 4.3.1.1 | H188A |
mutation of His188 to Ala only changes the active site structure and slightly elongates the distance of Cbeta proton of substrate with Ser318, causing the enzyme to remain significant but reduced activity |
-, 730225 |
| 4.3.1.1 | H188A |
responsible for binding the amino group of the substrate |
703646 |
| 4.3.1.1 | H188K |
responsible for binding the amino group of the substrate |
703646 |
| 4.3.1.1 | H188Q |
responsible for binding the amino group of the substrate |
703646 |
| 4.3.1.1 | H188R |
responsible for binding the amino group of the substrate |
703646 |
| 4.3.1.1 | I320A |
SS-loop mutant, about 2% of wild-type activity |
-, 714262 |
| 4.3.1.1 | K126R |
replacement of Lys126 with Arg increases the activity of the enzyme |
6023 |
| 4.3.1.1 | K140I |
Km value 10fold higher than wild type, comparable increase in Ki for competitive inhibitors |
649266 |
