EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.B68 | E179K |
site-directed mutagenesis, inactive mutant |
746752 |
| 4.2.3.B68 | E179Q |
site-directed mutagenesis, the mutation results in strongly reduced activity with both metal cations compared to wild-type |
-, 746752 |
| 4.2.3.B68 | E193D |
site-directed mutagenesis, the mutation results in reduced activity with Mg2+ compared to wild-type, or a complete loss of activity with Mn2+ |
746752 |
| 4.2.3.B68 | L72A |
site-directed mutagenesis, the mutant variant of enzyme SaS gives no soluble protein |
-, 746752 |
| 4.2.3.B68 | P201T |
site-directed mutagenesis, the mutant variant of SaS shows significantly reduced activity with both metal cofactors compared to wild-type |
746752 |
| 4.2.3.B68 | P65A |
site-directed mutagenesis, the mutation yields wild-type levels of enzyme, but the activity drops significantly with Mg2+ and Mn2+ |
746752 |
| 4.2.3.B68 | Q160E |
site-directed mutagenesis, the mutant enzyme is well expressed, but shows no activity with either Mg2+ or Mn2+ |
746752 |
| 4.2.3.B68 | R145K |
site-directed mutagenesis, the mutation results in reduced activity with Mg2+ compared to wild-type, or a complete loss of activity with Mn2+ |
-, 746752 |
| 4.2.3.B68 | R145M |
site-directed mutagenesis, the mutant variant shows only a slight decrease in activity for both metal cofactors compared to wild-type |
-, 746752 |
