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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 4.2.3.B66more construction of a recombinant gene where GFP is fused to the putative chloroplast transit peptide (cTP) sequence of OsKSL2. The fusion gene is transiently expressed in rice leaf protoplasts. GFP signals from the transformed cells overlap with autofluorescence from chloroplasts, indicating that OsKSL2 is localized to the plastid. The subcellular localization of the recombinant GFP-tagged enzyme with GFP fused to a shorter transit peptide sequence from gene Os04g0612000 (UniProt ID Q0JA81) in protoplast cells does not localize to the plastids, the shorter sequence fails to function as a transit peptide 746968
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