EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.95 | C236A |
the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% sesquiterpene products from (2E,6E)-farnesyl diphosphate (41.2% of wild type activity) |
713839 |
| 4.2.3.95 | E237L |
the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% unidentified sesquiterpene products from (2E,6E)-farnesyl diphosphate (59.4% of wild type activity) |
713839 |
| 4.2.3.95 | more |
in the Cop6 loop graft mutant Cop6L4, loop replacement has no influence on the selectivity of the enzyme for alpha-cuprenene, the mutation reduces the catalytic efficiency 3-17fold |
713839 |
| 4.2.3.95 | N224D |
the mutant makes a small amount of (6S)-beta-bisabolene (18%) from (2Z,6E)-farnesyl diphosphate, the mutation does not affect the product profile of Cop6 with its normal substrate (2E,6E)-farnesyl diphosphate |
714733 |
| 4.2.3.95 | N240L |
the mutant produces 98% (-)-alpha-cuprenene and 2% gamma-cuprenene from (2E,6E)-farnesyl diphosphate (31% of wild type activity) |
713839 |
