EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.87 | more |
the two polymorphisms within the active site of VvGuaS compared to VvPNSeInt (EC 4.2.3.86) correspond to T414S and V530M substitutions |
748331 |
| 4.2.3.87 | T414S |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
748331 |
| 4.2.3.87 | T414S/V530M |
site-directed mutagenesis, the mutant shows a guaiene synthase activity that is almost completely compromised, with only 5% of the volatile products comprising alpha-guaiene, and alpha-bulnesene not detectable. Instead, the major products are predominantly the 6,6 bicyclic sesquiterpenes selina-4,11-diene (40%), alpha-selinene (23%), epi-alpha-selinene (12%), and a hydroxylated selinene (9%) with the same reported RI as intermedeo |
748331 |
| 4.2.3.87 | V530M |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
748331 |
| 4.2.3.87 | W144R/V241E/P296S/H337Y |
inactive |
716607 |
| 4.2.3.87 | Y110S/V241E/P296S/H337Y |
the mutant produces 34.1% alpha-guaiene, 1.3% alpha-humulene and 64.6% delta-guaiene |
716607 |
| 4.2.3.87 | Y110S/W144R/P296S/H337Y |
inactive |
716607 |
| 4.2.3.87 | Y110S/W144R/V241E/H337Y |
inactive |
716607 |
| 4.2.3.87 | Y110S/W144R/V241E/P296S |
inactive |
716607 |
| 4.2.3.87 | Y110S/W144R/V241E/P296S/H337Y |
the mutant produces 31.1% alpha-guaiene, 1.5% alpha-humulene and 67.4% delta-guaiene |
716607 |
