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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.70more construction of several fusion protein variants in which farnesyl diphosphate synthase of yeast has been coupled to patchoulol synthase of plant origin. patchoulol synthase can be extended N- and C-terminally with farnesyl diphosphate synthase and retain activity in vivo. Expression of the fusion proteins in Saccharomyces cerevisiae increases the production of patchoulol up to 2-fold 710950
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.70more fusion to thioredoxin and careful codon optimization of the eukaryotic sequence improves solubl expression on average by 42% in comparison to an unoptimized, His-tagged construct 730883
Display the word mapDisplay the reaction diagram Show all sequences 4.2.3.70more use of a bifunctional enzymatic chimera composed as a fusion between farnesyl diphosphate synthase from yeast and patchoulol synthase from patchouli improves patchoulol production compared to that with the use of free enzymes up to 2fold. The effect is additive to improvements obtained by traditional metabolic engineering 710950
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