EC Number   |
Protein Variants |
Reference |
|---|
  4.2.3.42 | C831G |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
| 4.2.3.42 | C831T |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
| 4.2.3.42 | D661A |
site-directed mutagenesis, the mutation results in a premature termination of the cyclization reaction cascade en-route from syn-copalyl diphosphate to aphidicolan-16-beta-ol, the mutant generates the diterpene macrocycle syn-copalol and a minor, non-hydroxylated labdane related diterpene |
747838 |
| 4.2.3.42 | F629L |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
| 4.2.3.42 | F789L |
site-directed mutagenesis, inactive mutant |
747838 |
| 4.2.3.42 | I626A |
site-directed mutagenesis, inactive mutant |
747838 |
| 4.2.3.42 | T920G |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
| 4.2.3.42 | Y658F |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
| 4.2.3.42 | Y658L |
site-directed mutagenesis, the mutation results in a premature termination of the cyclization reaction cascade en-route from syn-copalyl diphosphate to aphidicolan-16-beta-ol, the mutant generates the diterpene macrocycle syn-copalol and a minor, non-hydroxylated labdane related diterpene |
747838 |
| 4.2.3.42 | Y923F |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
747838 |
