EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.30 | more |
of the three differences in active site residues between the orthologous OsKSL5j and OsKSL5i, switching the Ile at position 664 in OsKSL5i to the Thr found in OsKSL5j is sufficient to short circuit the production of ent-isokaurene, as OsKSL5i:I664T specifically produces ent-pimara-8(14),15-diene instead. But the reciprocal residue switch leads to production of a mixture of tetracycles ent-isokaurene, ent-kaurene, and ent-atiserene (i.e. by OsKSL5j:T664I), rather than the almost exclusive production of ent-isokaurene observed with OsKSL5i |
746596 |
| 4.2.3.30 | T664I |
site-directed mutagenesis, the OsKSL5j:T664I mutant produces only ent-pimara-8(14),15-diene |
746596 |
| 4.2.3.30 | T664I/L661V |
site-directed mutagenesis, the addition of L661Vto mutation T664I leads to much more specific production of ent-isokaurene, the product outcome mediated by this OsKSL5j:L661V/T664I double residue switch mutant is essentially identical to that mediated by OsKSL5i |
746596 |
| 4.2.3.30 | T664I/V718I |
site-directed mutagenesis, the addition of V718I to mutation T664I does not further alter product outcome |
746596 |
