EC Number |
Protein Variants |
Reference |
---|
4.2.3.135 | A159E |
site-directed mutagenesis of Omp7, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+ and Mn2+, slight to moderate reduction with Co2+, slightly reduced activity with Ni2+, and strong reduction of activity with Ca2+ |
747535 |
4.2.3.135 | D111A |
site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows moderately reduced activity with Mg2+, slight to moderate reduction of activity with Mn2+, strong reduction of activity with Co2+, and no activity with Ca2+ and Ni2+ |
747535 |
4.2.3.135 | E117A |
site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight to moderate reduction of activity with Mg2+, strongly reduced activity with Ca2+ and Mn2+, very strong reduction of activity with Co2+, and no activity with Ni2+ |
747535 |
4.2.3.135 | E164A |
site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+, strong reduction with Ca2+, Co2+, and Mn2+, and very strong reduction with Ni2+ |
747535 |
4.2.3.135 | E187A |
site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight to moderate reduction of activity with Mg2+, and no activity with Ca2+, Mn2+, Ni2+, and Co2+ |
747535 |
4.2.3.135 | E53A |
site-directed mutagenesis of Stehi73029, compared to wild-type, the mutant shows slight reduction of activity with Mg2+, moderately reduced activity with Ca2+ and Mn2+, and no activity with Ni2+ and Co2+ |
747535 |
4.2.3.135 | more |
coexpression of protoilludene synthase PpSTS-08 and diverse PpCYPs in Saccharomyces cerevisiae by chromosomal integration of the PpSTS-08 expression cassette and subsequent transformation with expression plasmids of 184 PpCYPs previously constructed resulting in 184 distinct transformants that give simultaneous expression of PpSTS-08 with each of the PpCYPs |
748574 |
4.2.3.135 | more |
protoilludene is successfully produced in Escherichia coli by overexpression of a hybrid exogenous mevalonate pathway, endogenous farnesyl diphosphate synthase (IspA), and protoilludene synthase (OMP7) of Omphalotus olearius |
748585 |
4.2.3.135 | S93E/A159E |
site-directed mutagenesis of Omp7, compared to wild-type, the mutant shows moderate reduction of activity with Co2+, Mg2+, and Mn2+, slightly reduced activity with Ni2+, and strong reduction of activity with Ca2+ |
747535 |
4.2.3.135 | T165A |
site-directed mutagenesis of Omp6, compared to wild-type, the mutant shows moderate reduction of activity with Mg2+ and Ni2+, slight to moderate reduction with Co2+, slightly reduced activity with Mn2+, and very strong reduction of activity with Ca2+ |
747535 |