EC Number   |
Protein Variants |
Reference |
|---|
    4.2.3.124 | E243Q |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
719072 |
| 4.2.3.124 | K141Q |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
719072 |
| 4.2.3.124 | more |
complementation of a btrC2 disruption mutant by expression of wild-type btrC2 from plasmid pHB201 in Bacillus circulans |
-, 719115 |
| 4.2.3.124 | more |
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of a natural btrC gene, encoding DOI synthase in butirosin-producing Bacillus circulans, in the heterologous host Bacillus subtilis strain 168-BSDOI-2 generates approximately 2.3 g/l 2-deoxy-scyllo-inosose (DOI), fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production |
747856 |
| 4.2.3.124 | more |
construction of several metabolically engineered recombinant strains of Bacillus subtilis, and comparison of the DOI titer produced by the recombinants that express a heterologous DOI synthase. The expression of gene btrC encoding DOI synthase derived from tobramycin-producing Streptomyces tenebrarius in recombinant of Bacillus subtilis strain BSDOI-15, in which both genes pgi and pgcA are disrupted, significantly enhances the 2-deoxy-scyllo-inosose (DOI) titer to up to 37.2 g/l, fed-batch fermentation, UPLC-ESI-MS/MS analysis of DOI production |
-, 747856 |
| 4.2.3.124 | more |
disruption of gene btrC2 by insertion of a tetracycline resistance cassette |
-, 719110 |
| 4.2.3.124 | more |
introduction of DOIS gene btrC from Bacillus circulans and disruption of genes for phosphoglucose isomerase, D-glucose 6-phosphate dehydrogenase, and phosphoglucomutase, pgi, zwf and pgm, respectively, in Escherichia coli strain GI724. While GI724DELTAP/pGA-btrC and GI724DELTAPP/pGA-btrC possess functional Zwf and thus can catabolize glucose via the pentose phosphate pathway, glucose catabolism is completely inhibited in GI724DELTAPZ/pGA-btrC and GI724DELTAPZP/pGA-btrC due to the deletion of both pgi and zwf genes |
-, 720081 |
| 4.2.3.124 | more |
transgenic production of 2-deoxy-scyllo-inosose (DOI) in cyanobacterium Synechococcus elongatus strain PCC 7942, production of 400 mg/l of DOI without using an external carbon source, method overview |
747325 |
