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Results 1 - 10 of 10
EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 4.2.3.109A595G shift in product profile, mutant produces 2.4% (+)-car-3-ene, 39% (-)-sabinene, 33.3% terpinolene 730053
Display the reaction diagram Show all sequences 4.2.3.109A595G/F596L/L599F shift in product profile, mutant produces 42.3% (+)-car-3-ene, 7.3% (-)-sabinene, 20.1% terpinolene 730053
Display the reaction diagram Show all sequences 4.2.3.109F596E shift in product profile, mutant produces 0.5% (+)-car-3-ene, 10.2% (-)-sabinene, 5.7% terpinolene, 70.9% limonene 730053
Display the reaction diagram Show all sequences 4.2.3.109F596G shift in product profile, mutant produces 10.0% (+)-car-3-ene, 11.2% (-)-sabinene, 17.3% terpinolene, 27.0% myrcene, 17.0% limonene 730053
Display the reaction diagram Show all sequences 4.2.3.109F596H shift in product profile, mutant produces 2.1% (+)-car-3-ene, 46.5% (-)-sabinene, 27.1% terpinolene 730053
Display the reaction diagram Show all sequences 4.2.3.109F596L shift in product profile, mutant produces 28.6% (+)-car-3-ene, 42.8% (-)-sabinene, 36.4% terpinolene 730053
Display the reaction diagram Show all sequences 4.2.3.109F596R inactive 730053
Display the reaction diagram Show all sequences 4.2.3.109L599F shift in product profile, mutant produces 1.5% (+)-car-3-ene, 20.2% (-)-sabinene, 32.2% terpinolene 730053
Display the reaction diagram Show all sequences 4.2.3.109more construction of a series of domain swaps and site-directed substitutions between (-)-sabinene synthase and (+)-3-carene synthase isoforms to explore which regions and specific amino acids of these enzymes affect the differences of their product profiles. The amino acid in position 596 is critical for product profiles dominated by or (-)-sabinene. A leucine in this position promotes formation of (+)-3-carene, whereas phenylalanine promotes (-)-sabinene 730053
Display the reaction diagram Show all sequences 4.2.3.109S589A shift in product profile, mutant produces 1.2% (+)-car-3-ene, 42.9% (-)-sabinene, 35.5% terpinolene 730053
Results 1 - 10 of 10