EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.108 | F266C |
site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme |
748984 |
| 4.2.3.108 | F266S |
site-directed mutagenesis, the mutation shifts the the product spectrum significantly toward alpha-terpineol compared to wild-type enzyme |
748984 |
| 4.2.3.108 | F266T |
site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme |
748984 |
| 4.2.3.108 | F266V |
site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme |
748984 |
| 4.2.3.108 | F266Y |
site-directed mutagenesis, the mutation only slightly alters the product spectrum of the mutant enzyme compared to wild-type enzyme |
748984 |
| 4.2.3.108 | more |
although the distributions of the five detectable products sabinene, beta-myrcene, limonene, 1,8-cineole, and alpha-terpineol change slightly in all these mutants compared to wild-type, none of them shifts the product spectrum significantly toward alpha-terpineol, with exception of mutant F266S, overview |
748984 |
| 4.2.3.108 | more |
conversion of CinS1 to a sabinene synthase based on an amino acid comparison of the enzyme with Salvia officinalis SabS1 and Salvia pomifera SabS1 |
718210 |
| 4.2.3.108 | more |
exchange of sequences corresponding by homology to the C-terminal domain including the domain spanning alpha-helix, between sabinene synthase and both bornyl diphosphate synthase and cineole synthase. Exchange of residues 304-377 from cineole synthase into sabinene synthase is sufficient to impart the alternative termination chemistry involving water capture to alpha-terpineol and cyclization to 1,8-cineole |
717153 |
| 4.2.3.108 | more |
the N-terminal signal peptide of AaTPS6 (46 amino acid residues) before RR motif is truncated and recombinantly expressed in Escherichia coli strain BL21(DE3) |
747873 |
| 4.2.3.108 | N338A |
products are 11.7% alpha-pinene, 33.1% sabinene, 33.2% beta-pinene, 16.9% myrcene, 5.1% limonene |
718210 |
