EC Number   |
Protein Variants |
Reference |
|---|
    4.2.2.5 | E349A |
no residual activity |
-, 747941 |
| 4.2.2.5 | H203A |
no residual activity |
-, 747941 |
| 4.2.2.5 | H225A |
site-directed mutagenesis, active site residue, inactive mutant |
650050 |
| 4.2.2.5 | I236T |
mutation introduced to render the enzyme identical to ACII from Arthrobacter aurescens IAM 110 65. Mutant behaves similar to wild-type |
-, 747368 |
| 4.2.2.5 | more |
a vector is engineered containing the gene for Flavobacterium heparinum chondroitinase AC for expression in adult bone marrow-derived cells which are then transplanted into an injury site in the CNS. Expression and secretion of active chondroitinase AC is observed in vitro using transfected Chinese hamster ovarian and gliosarcoma cells and in vivo by immunohistochemistry analysis which show degraded chondroitin sulfate coinciding with the location of transfected bone marrow-derived cells. Immunolabelling of the axonal growth-associated protein GAP-43 is observed in vivo and coincides with the location of degraded chondroitin sulfate |
693771 |
| 4.2.2.5 | N153A |
no residual activity |
-, 747941 |
| 4.2.2.5 | R266A |
no residual activity |
-, 747941 |
| 4.2.2.5 | R288A |
site-directed mutagenesis, active site residue, nearly inactive mutant |
650050 |
| 4.2.2.5 | R292A |
site-directed mutagenesis, substrate recognition residue, mutant with residual activity |
650050 |
| 4.2.2.5 | R292A |
site-directed mutagenesis, substrate recognition residue, reduced activity, action pattern is neither purely random endolytic nor purely random exolytic |
650383 |
