EC Number   |
Protein Variants |
Reference |
|---|
   4.2.2.26 | H178A |
mutant shows reduced oligoalginate lyase activity |
-, 734607 |
| 4.2.2.26 | H208F |
in addition to cleaving alginate-based substrates, mutant displays significant exolytic glucuronan activity |
-, 747352 |
| 4.2.2.26 | H389A |
mutant shows reduced oligoalginate lyase activity |
734607 |
| 4.2.2.26 | N177A |
mutant shows reduced oligoalginate lyase activity |
-, 734607 |
| 4.2.2.26 | N207L |
completely inactive |
-, 747352 |
| 4.2.2.26 | R236A |
mutant exhibits endolytic activity. The overexpressed mutant protein (79.6 kDa) is proteolytically cleaved into the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both fragments show endolytic lyase activity |
-, 734607 |
| 4.2.2.26 | Y234F |
mutant shows reduced oligoalginate lyase activity |
-, 734607 |
| 4.2.2.26 | Y264F |
completely inactive |
-, 747352 |
| 4.2.2.26 | Y426F |
mutant shows reduced oligoalginate lyase activity |
-, 734607 |
| 4.2.2.26 | Y455F |
significantly diminished yet measurable activity toward alginate and M and G blocks |
-, 747352 |
