EC Number   |
Protein Variants |
Reference |
|---|
   4.2.2.18 | D233N |
inactive mutant enzyme |
-, 680892 |
| 4.2.2.18 | E244Q |
inactive mutant enzyme |
-, 680892 |
| 4.2.2.18 | G221R |
point-mutation introduced via error-prone PCR, increase in activity of 35% |
644471 |
| 4.2.2.18 | more |
genetic engineering of the enzyme for enlarged thermotolerance in large scale production of DFA III, recombinant expression in Escherichia coli, elimination of sequence coding for a signal-peptide: 18% secretion, 82% intracellular localisation of the enzyme in Escherichia coli |
644471 |
| 4.2.2.18 | more |
immobilization of enzyme on alginate beads lowered the activity |
644471 |
| 4.2.2.18 | S309A |
95.8% of wild-type activity, 17.9 degrees decrease in melting temperature |
748006 |
| 4.2.2.18 | S309A/S333A |
88.2% of wild-type activity, 25.9 degrees decrease in melting temperature |
748006 |
| 4.2.2.18 | S309C |
94.9% of wild-type activity, 16.8 degrees decrease in melting temperature |
748006 |
| 4.2.2.18 | S309C/S333C |
84.5% of wild-type activity, 23.7 degrees decrease in melting temperature |
748006 |
| 4.2.2.18 | S309F |
102.6% of wild-type activity, 15.8 degrees decrease in melting temperature |
748006 |
