EC Number   |
Protein Variants |
Reference |
|---|
   4.2.1.94 | F162A |
crystal structure |
648512 |
| 4.2.1.94 | more |
C-terminal deletion mutants: deletion of 6 amino acids, 95% loss of activity |
648515 |
| 4.2.1.94 | more |
fungal isolates resistant to the melanin biosynthesis inhibitors-dehydratase (MBI-D), which inhibits scytalone dehydratase, are detected in Japan. The isolates have a single point mutation in the gene encoding scytalone dehydratase. In fitness tests, resistant isolates show reduced fitness, compared with that of sensitive isolates. Enzyme kinetics analyses of the scytalone dehydratases from MBI-D-sensitive and MBI-D-resistant isolates reveal that the activity of MBI-D-sensitive-type enzymes is approximately double that of MBI-D-resistant-type enzymes. The sensitive-type enzyme has higher scytalone dehydration activity than that of resistant-type enzyme at temperatures ranging from 15-35°C and at pHs ranging from pH 6.0-9.0. Acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity |
748472 |
| 4.2.1.94 | more |
gene SCD1 encoding a scytalone dehydratase is disrupted by split-marker target gene replacement, protoplast transformation. Disruption of SCD1 does not change the pathogenicity of the fungus, but results in slower radial growth, less biomass, wider angled hyphal branches, impaired sclerotial development and decreased resistance to UV light. All gene knockout strains cause pathogenicity symptoms similar to those of the wild-type with both inoculation methods |
-, 748709 |
| 4.2.1.94 | more |
inhibitors that display variations in the hydrophilic region of the SD binding site, kinetic data for wild type and mutant enzymes |
648510 |
| 4.2.1.94 | more |
kinetic data of several point mutants |
648507, 648511 |
| 4.2.1.94 | V75M |
inhibition of the mutant enzyme by (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide is more than 200fold reduced in comparison with that of the wild-type, about 2fold reduced turnover number for scytalone as compared to wild-type enzyme |
661361 |
