EC Number |
Protein Variants |
Reference |
---|
4.2.1.51 | C216A |
site-directed mutagenesis, inactive mutant |
649980 |
4.2.1.51 | C216S |
site-directed mutagenesis, increased activity |
649980 |
4.2.1.51 | E159A |
site-directed mutagenesis, 2.2fold increased activity |
649980 |
4.2.1.51 | E159A/E232A |
site-directed mutagenesis, 7fold increased kcat, 4.6fold decreased Km compared to the wild-type, increased activity |
649980 |
4.2.1.51 | E232A |
site-directed mutagenesis, 3.5fold increased activity |
649980 |
4.2.1.51 | E64D |
constructed by PCR-based random mutagenesis and complementation analysis, 15% reduced activity compared to the wild-type enzyme, 4.5% increased Km, 1.7fold increased kcat |
649516 |
4.2.1.51 | E64Q |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
4.2.1.51 | E64S |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
4.2.1.51 | E64V |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |
4.2.1.51 | F185L |
constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant |
649516 |