  4.2.1.135 | more |
four genes, Pdeg (UDP-N-acetylglucosamine C4,6-dehydratase), Preq (UDP-4-reductase), UDP-GlcNAc 6-DH (UDP-N-acetylglucosamine 6-dehydrogenase), and UXNAcS (UDP-N-acetylxylosamine synthase), are employed to synthesize UDP-quinovosamine, UDP-N-acetylglucosaminuronic acid, and UDP-N-acetylxylosamine in Escherichia coli. Escherichia coli nucleotide sugar biosynthetic pathway is engineered to increase the pool of substrate for the target nucleotide sugars. Uridine diphosphate dependent glycosyltransferase (UGT) is also selected and introduced into Escherichia coli. High levels of three novel flavonoid glycosides are obtained in the engineered mutant with 158.3 mg/l quercetin 3-O-(N-acetyl) quinovosamine, 172.5 mg/l luteolin 7-O-(N-acetyl)glucosaminuronic acid, and 160.8 mg/l quercetin 3-O-(N-acetyl)xylosamine |
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