EC Number   |
Protein Variants |
Reference |
|---|
   4.2.1.131 | D266A |
site-directed mutagenesis, inactive mutant |
746852 |
| 4.2.1.131 | D268A |
site-directed mutagenesis, inactive mutant |
746852 |
| 4.2.1.131 | H237A |
site-directed mutagenesis, inactive mutant |
746852 |
| 4.2.1.131 | H239A |
site-directed mutagenesis, inactive mutant |
746852 |
| 4.2.1.131 | H262A |
site-directed mutagenesis, the mutant shows highly increased activity compared to the wild-type enzyme |
746852 |
| 4.2.1.131 | H264A |
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme |
746852 |
| 4.2.1.131 | more |
expression levels of wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), overview. While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein. The N-terminally trubcated mutant also shows increased activity compared to the wild-type enzyme |
746852 |
| 4.2.1.131 | more |
expression levels of wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), overview. While the removal of the N-terminus result in an increased expression level, all point mutations negatively influences the expression of the protein. The N-terminally truncated mutant also shows increased activity compared to the wild-type enzyme |
746852 |
| 4.2.1.131 | S58Q |
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme |
746852 |
| 4.2.1.131 | S58V |
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme |
746852 |
