EC Number |
Protein Variants |
Reference |
---|
4.2.1.125 | C264A |
site-directed mutagenesis, the mutant shows 53% decreased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | C489A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | C568A |
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | D488A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | F477A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | I559A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
4.2.1.125 | more |
establishment of dammarane-type ginsenosides synthesis from 2,3-oxidosqualene in Oryza sativa by expression of dammarenediol-II synthase from Panax ginseng resulting in a dammarane-type sapogenin 20(S)-protopanaxadiol (PPD) content of 0.35-0.59 mg/gdw and a dammarane-type sapogenin 20(S)-protopanaxatriol (PPT) content of 0.23-0.43 mg/g dry weight in the transgenic rice plants |
749016 |
4.2.1.125 | more |
expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase results in production of dammarenediol and hydroxydammarenone. Silencing of gene expression by RNAi leads to a reduction of ginsenoide production to 84.5% in roots |
683979 |
4.2.1.125 | more |
functionally expressed in the lanosterol synthase-deficient yeast mutant erg7 |
710295 |
4.2.1.125 | more |
heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview |
747390 |