EC Number |
Protein Variants |
Reference |
---|
4.1.99.22 | C24S/C28S/C31S |
the mutant does not contain the catalytic S-adenosyl-L-methionine-binding cluster I |
715257 |
4.1.99.22 | D198A |
mutation in GG motif, decrease in affinity for S-adenosine-L-methionine |
-, 738455 |
4.1.99.22 | G339A |
mutation in GG motif, loss of activity |
-, 738455 |
4.1.99.22 | G340A |
mutation in GG motif, loss of activity |
-, 738455 |
4.1.99.22 | more |
deletion of central components of the ISC system in addition to IscS leads to an overall decrease in Fe-S cluster enzyme and molybdoenzyme activity in addition to a decrease in the number of Fe-S-dependent thiomodifications of tRNA, based on the fact that some proteins involved in Moco biosynthesis and tRNA thiolation are Fe-S-dependent. Complementation of the ISC deficient strains with the suf operon restores the activity of Fe-S-containing proteins, including the MoaA protein, which is involved in the conversion of 5'-GTP to cyclic pyranopterin monophosphate in the fist step of Moco biosynthesis |
-, 747120 |
4.1.99.22 | more |
generation of cnx2 mutants by T-DNA insertion, line cnx2-1, and point mutation, line cnx2-2. Mutation mapping by whole genome sequencing, phenotype analyses |
-, 747034 |
4.1.99.22 | N124A/N165A |
mutation reduces binding of 5'-GTP |
716738 |
4.1.99.22 | R17A |
complete loss of activity |
716738 |
4.1.99.22 | R17A/R266A/R268A |
complete loss of activity |
716738 |
4.1.99.22 | R192A |
80% loss of activity |
716738 |