EC Number   |
Protein Variants |
Reference |
|---|
    4.1.2.55 | T157C |
improved for 2-dehydro-3-deoxy-D-gluconate with a 75% diastereoisomeric ratio |
715259 |
| 4.1.2.55 | T157C/Y132V |
diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (91%) diastereoisomeric ratio |
715259 |
| 4.1.2.55 | T157F/Y132V |
diastereoselective formation of 2-dehydro-3-deoxy-D-gluconate with much improved (93%) diastereoisomeric ratio |
715259 |
| 4.1.2.55 | T157V/A198L/D181Q |
diastereoselective formation of 2-dehydro-3-deoxy-D-galactonate with much improved (88%) diastereoisomeric ratio |
715259 |
| 4.1.2.55 | V193A |
the mutant enzyme displays a threefold increase in activity compared with wild type enzyme. Increased specific activity at 4060 °C of this mutant is observed, not only for the condensation of pyruvate with glyceraldehyde, but also for several unnatural acceptor aldehydes. The optimal temperature for activity of SacKdgAV193A is lower than for the wild type enzyme, but enzymatic stability of the mutant is similar to that of the wild type |
718713 |
