EC Number   |
Protein Variants |
Reference |
|---|
   4.1.2.49 | D168N |
mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme |
747787 |
| 4.1.2.49 | D168S |
mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme |
747787 |
| 4.1.2.49 | D168T |
mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme |
747787 |
| 4.1.2.49 | D168V |
mutation reduces the specific activity, which is partially restored by the addition of excess pyridoxal 5'-phosphate to the reaction mixture. The mutant enzyme is able to catalyse the aldol synthesis of L-beta-phenylserine from benzaldehyde and glycine with yields similar to the wild-type enzyme |
747787 |
| 4.1.2.49 | H128Y |
8.4fold increase in activity towards L-threonine and a 2.0fold increase towards L-allo-threonine compared with the wild-type enzyme |
-, 746614 |
| 4.1.2.49 | H128Y/S292R |
3fold and 322fold increased kcat/Km values towards towards L-allo-threonine and L-threonine compared with the wild-type enzyme |
-, 746614 |
| 4.1.2.49 | K199A |
catalytically inactive mutant enzyme |
-, 715334 |
| 4.1.2.49 | K199R |
mutation reduces the specific activity of the enzyme by 5000fold, which can be partially restored by adding an excess of pyridoxal 5'-phosphate to the reaction medium |
747787 |
| 4.1.2.49 | K224A |
mutant enzyme shows properties similar to the wild type enzyme |
-, 715334 |
| 4.1.2.49 | K51A |
mutant enzyme shows properties similar to the wild type enzyme |
-, 715334 |
