EC Number |
Protein Variants |
Reference |
---|
4.1.2.40 | K124A |
strongly reduced aldolase activity |
679492 |
4.1.2.40 | K204A |
mutation results in more than 98% decrease in aldolase activity |
679492 |
4.1.2.40 | L165E |
the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate |
715040 |
4.1.2.40 | L165E/L275S |
the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate |
715040 |
4.1.2.40 | L275S |
the mutant shows enhanced substrate specificity toward fructose-1,6-bisphosphate |
715040 |
4.1.2.40 | more |
construction of chimeras of isoforms LacD.1 and LacD.2. The C-terminus of LacD.1 contributes to its differential enzymatic activity, as replacing of its C-terminal half with 162 amino acid residues from the C-terminus of LacD.2 leads to an enzyme very similarly to LacD.2 in the cleavage of tagatose bisphosphate but over 3 times more efficient than LacD.2 at cleaving fructose bisphosphate. In addition, loop 1 and turn 2 influence the differential enzymatic activity of LacD.1 |
-, 727768 |