EC Number |
Protein Variants |
Reference |
---|
4.1.2.38 | A28S |
mutant shows decarboxylase activity towards 2-keto acids |
678578 |
4.1.2.38 | A28S |
point mutation converts BAL into a true benzoylformate decarboxylase. Km value for (R)-benzoin is virtually identical to that of the wild-type enzyme but whose kcat value is reduced ca. 10fold. Can decarboxylate benzoylformate, has a substrate spectrum comparable to that of benzoylformate decarboxylase, albeit with reduced activity. Methyl benzoylphosphonate and benzoylphosphonate act as competitive inhibitors in a time- and concentration-dependent manner |
704205 |
4.1.2.38 | A480I |
reduced lyase activity |
678578 |
4.1.2.38 | F484I |
reduced lyase activity |
678578 |
4.1.2.38 | H286A |
reduced lyase activity |
678578 |
4.1.2.38 | H29A |
the mutant shows 2.7% of wild type activity |
747990 |
4.1.2.38 | H29A |
the role of His29 in cofactor activation and catalysis is delineated |
690982 |
4.1.2.38 | Q112A/E393A/S416G/T479A |
variant with larger but less polar binding site |
-, 762916 |
4.1.2.38 | Q112L/E393Y/S416G/T479F |
variant with binding site matched to Pseudomonas fluorescens enzyme |
-, 762916 |
4.1.2.38 | W163A |
the role of Trp163 in cofactor activation and catalysis is delineated |
690982 |