EC Number   |
Protein Variants |
Reference |
|---|
    4.1.2.14 | E9K/V118A |
KA1-4 variant, improved cleavage of D-2-dehydro-deoxygluconate |
650823 |
| 4.1.2.14 | G40A/V188A |
KA1-1 variant, improved cleavage of D-2-dehydro-deoxygluconate |
650823 |
| 4.1.2.14 | K133Q |
no aldolase activity |
653924 |
| 4.1.2.14 | K133Q/T161K |
reduced catalytic efficiency |
653924 |
| 4.1.2.14 | S184A |
the mutation decreases the efficiency of KDPG retro-aldol cleavage only modestly |
682736 |
| 4.1.2.14 | S184D |
the mutation leads to strongly reduced enzymatic activity |
682736 |
| 4.1.2.14 | S184F |
active site mutation, enhances the substrate specificity to include catalysis of retro-aldol cleavage of (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butanoate |
726975 |
| 4.1.2.14 | S184L |
the mutation is located in the substrate-binding pocket, interacts with the phosphate moiety of KDPG, and improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40fold |
682736 |
| 4.1.2.14 | T161A |
strongly reduced activity |
678625 |
| 4.1.2.14 | T161S |
active site mutation, enhances the substrate specificity to include catalysis of retro-aldol cleavage of (4S)-2-keto-4-hydroxy-4-(2'-pyridyl)butanoate |
726975 |
