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EC Number
Protein Variants
Commentary
Reference
4.1.1.98
V47S
a FMN-free mutant
728916
4.1.1.98
V47S
complete loss in FMN binding, crystal structure
749310
4.1.1.98
V47S
site-directed mutagenesis, the single mutation results in a loss of FMN binding, resulting in the production of FMN-free CpsUbiX protein. The mutation is likely to destabilize FMN-protein interactions without affecting the overall structural folding. The mutant enzyme has an FMN:protein molar ratio of 0.086:1. Wild-type CpsUbiX protein has a yellowish colour, while the V47S mutant does not
746643
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