EC Number |
Protein Variants |
Reference |
---|
3.8.1.8 | A170T |
naturally occuring mutation G508A, the mutation confers higher specificity for simazine, albeit not atrazine, and greater affinity for a metal ion required for activity |
733440 |
3.8.1.8 | A170T/M256I/P258T/Y261S |
commercially prepared mutant gene |
733014 |
3.8.1.8 | A216A/T217D/T219E/A220A/D250D |
site-directed mutagenesis, AtzA-variant 288. Km 49 microM, kcat 4.3 s-1, kcat/Km 88000 s-1*M-1 |
-, 690560 |
3.8.1.8 | A216A/T217S/T219H/A220G/D250Y |
site-directed mutagenesis, AtzA-variant 431. Km 76 microM, kcat 8.0 s-1, kcat/Km 110000 s-1*M-1 |
690560 |
3.8.1.8 | A216G/T217D/T219A/A220V/D250W |
AtzA mutant |
690560 |
3.8.1.8 | A216G/T217D/T219A/A220V/D250W |
site-directed mutagenesis, AtzA-variant 305. Km 95 microM, kcat 6.2 s-1, kcat/Km 65000 s-1*M-1 |
-, 690560 |
3.8.1.8 | A216G/T217D/T219G/A220H/D250D |
AtzA mutant |
690560 |
3.8.1.8 | A216G/T217D/T219G/A220H/D250D |
site-directed mutagenesis, AtzA-variant 734. Km 62 microM, kcat 15.1 s-1, kcat/Km 240000 s-1*M-1 |
690560 |
3.8.1.8 | A216G/T217D/T219G/A220H/D250G |
AtzA mutant |
690560 |
3.8.1.8 | A216G/T217D/T219G/A220H/D250G |
site-directed mutagenesis, AtzA-variant 430. Km 90 microM, kcat 12.7 s-1, kcat/Km 140000 s-1*M-1 |
690560 |