EC Number   |
Protein Variants |
Reference |
|---|
   3.6.4.B7 | C13A |
site-directed mutagenesis of the tetracysteine motif, the mutant variant binds ssDNA, and this interaction stimulates its ATPase activity. Wild-type RadA interacts with and inhibits the ATPase activity of RecA, but mutant RadA C13A fails to do so |
-, 757864 |
| 3.6.4.B7 | C13R |
site-directed mutagenesis of the tetracysteine motif, the mutant variant binds ssDNA, and this interaction stimulates its ATPase activity |
-, 757864 |
| 3.6.4.B7 | C28Y |
RadA mutant C28Y retains the ATPase activity but is defective in DNA binding |
756571 |
| 3.6.4.B7 | D246A |
loss of binding a second Mg2+. Initial ATP turnover rate is reduced by about 20-fold |
724274 |
| 3.6.4.B7 | D246N |
initial ATP turnover rate is reduced by about 20-fold |
724274 |
| 3.6.4.B7 | D302K |
mutant protein shows comparable strand exchange efficiencies in the presence of either potassium or sodium |
725699 |
| 3.6.4.B7 | E151D |
mutant protein retains potassium preference in promoting strand exchange. Reduced ATPase activity and normal strand exchange activity |
725699 |
| 3.6.4.B7 | E151K |
mutant protein retains potassium preference in promoting strand exchange. Reduced ATPase activity and normal strand exchange activity |
725699 |
| 3.6.4.B7 | E354A |
site-directed mutagenesis, mutant M9, matuation of a residue involved in ATPase function, and coordination of the nucleophilic water molecule |
-, 757833 |
| 3.6.4.B7 | E360A |
site-directed mutagenesis, mutant M5, mutation of residues involved in exstein-intein interaction |
-, 757833 |
