EC Number   |
Protein Variants |
Reference |
|---|
   3.6.1.39 | E343D |
the mutant does not hydrolyze dTTP |
719939 |
| 3.6.1.39 | E343N |
the mutant does not hydrolyze dTTP |
719939 |
| 3.6.1.39 | E343Q |
site-directed mutagenesis, the mutant is inactive, dTTP hydrolysis and DNA unwinding activities are abolished, but the mutant shows increased single-stranded DNA binding affinity compared to the wild-type enzyme, overview |
688933 |
| 3.6.1.39 | E343Q |
the mutant does not hydrolyze dTTP |
719939 |
| 3.6.1.39 | N468A |
the mutant hydrolyzes dTTP at rates comparable with wild type enzyme. N468A hydrolyzes nearly 4fold less efficiently than the wild type enzyme in the absence of single-stranded DNA and is not stimulated by the presence of single-stranded DNA |
719939 |
| 3.6.1.39 | R493A/N468A |
the mutant hydrolyzes dTTP at rates comparable with wild type enzyme and is not stimulated by the presence of single-stranded DNA |
719939 |
| 3.6.1.39 | R493Q |
the mutant hydrolyzes dTTP at rates comparable with wild type enzyme and is not stimulated by the presence of single-stranded DNA |
719939 |
| 3.6.1.39 | R493Q |
the mutant maintains only the basal level of dTTPase activity but does not exhibit activation in the dTTP hydrolysis activity in the presence of single-stranded DNA |
719939 |
