EC Number   |
Protein Variants |
Reference |
|---|
    3.5.4.9 | C58Y |
kcat/Km for 5,10-methenyltetrahydrofolate is 13.2fold lower than wild-type value |
744335 |
| 3.5.4.9 | D121A |
kcat/Km for 5,10-methenyltetrahydrofolate is 2348fold lower than wild-type value |
-, 744335 |
| 3.5.4.9 | D125A |
site-directed mutagenesis, inactive mutant |
656050 |
| 3.5.4.9 | D125C |
site-directed mutagenesis, inactive mutant |
656050 |
| 3.5.4.9 | D125E |
site-directed mutagenesis, 95% reduced activity in the forward reaction compared to the wild-type enzyme |
656050 |
| 3.5.4.9 | D125M |
site-directed mutagenesis, inactive mutant |
656050 |
| 3.5.4.9 | D125N |
site-directed mutagenesis, inactive mutant |
656050 |
| 3.5.4.9 | D125Q |
site-directed mutagenesis, inactive mutant |
656050 |
| 3.5.4.9 | G122D |
kcat/Km for 5,10-methenyltetrahydrofolate is 283fold lower than wild-type value |
-, 744335 |
| 3.5.4.9 | K54S |
very low activity with 5,10-methenyltetrahydrofolate |
-, 744335 |
