EC Number |
Protein Variants |
Reference |
---|
3.5.2.5 | D315A |
complete loss of activity |
720981 |
3.5.2.5 | D315N |
no activity |
699573 |
3.5.2.5 | D319A |
site-directed mutagenesis, inactive mutant, altered thermal denaturation compared to the wild-type enzyme |
-, 733438 |
3.5.2.5 | D319N |
site-directed mutagenesis, inactive mutant, altered thermal denaturation compared to the wild-type enzyme |
-, 733438 |
3.5.2.5 | H186A |
complete loss of activity |
720981 |
3.5.2.5 | H242A |
complete loss of activity |
720981 |
3.5.2.5 | H59A |
complete loss of activity |
720981 |
3.5.2.5 | H61A |
complete loss of activity |
720981 |
3.5.2.5 | K146A |
complete loss of activity |
720981 |
3.5.2.5 | more |
a T-DNA insertion allantoinase mutant is unable to grow on 10 mM allantoin as the sole nitrogen source, although it still shows some root elongation with a few plants reaching the four-leaf stage, the mutant is rescued by a C-terminally tagged Arabidopsis thaliana AtAln variant, overview |
689608 |