EC Number   |
Protein Variants |
Reference |
|---|
   3.5.2.12 | C316A |
18% of wild-type hydrolytic activity, decrease in the Km to approximately one-quarter the level of wild-type NylA, CD spectra similar to wild-type |
712418 |
| 3.5.2.12 | C316D |
1.5% of wild-type activity |
712418 |
| 3.5.2.12 | C316E |
1%% of wild-type activity |
712418 |
| 3.5.2.12 | C316G |
185% of wild-type activity |
712418 |
| 3.5.2.12 | C316K |
3.6% of wild-type activity |
712418 |
| 3.5.2.12 | C316N |
4.3% of wild-type activity |
712418 |
| 3.5.2.12 | C316S |
107% of wild-type activity |
712418 |
| 3.5.2.12 | K72A |
decreases the hydrolytic and esterolytic activities to undetectable levels |
712418 |
| 3.5.2.12 | N125A |
14% of wild-type hydrolytic activity, CD spectra similar to wild-type |
712418 |
| 3.5.2.12 | S150A |
decreases the hydrolytic and esterolytic activities to undetectable levels |
712418 |
