   3.5.1.91 | more |
development of six inducible shuttle vectors, pESH19cF, pESH19cR, pESH19kF, pESH19kR, pESH19aF, and pESH19aR, for Streptomyces-Escherichia coli, evaluation of stability and activity in expression of different enzymes including the N-substituted formamide deformylase as model enzymes, overview. Catechol 2,3-dioxygenase, nitrilase, and N-substituted formamide deformylase encoding genes are expressed from the different vectors as reporter genes showing that pESH19cF, pESH19kF, and pESH19aF possess inducible expression ability in Streptomyces lividans strain TK24. The stability test shows that the pSH19-derived shuttle vectors are stable in Escherichia coli strain Stbl2 and Streptomyces lividans strain TK24. Streptomyces lividans strain TK24 is the better host |
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