EC Number   |
Protein Variants |
Reference |
|---|
   3.5.1.81 | C96D |
inactive mutant enzymes |
-, 656205 |
| 3.5.1.81 | D366A |
inactive mutant enzymes |
-, 656205 |
| 3.5.1.81 | F191W |
site-directed mutagenesis, the catalytic efficiency of the mutant toward N-acetyl-D-Trp and N-acetyl-D-Ala is enhanced by 15.6 and 1.5folds, respectively, compared to the wild-type enzyme, with unaltered other properties, e.g. pH and temperature dependence |
-, 757308 |
| 3.5.1.81 | F191W |
the catalytic efficiency of the mutant toward N-acetyl-D-Trp and N-acetyl-D-Ala is enhanced by 15.6 and 1.5folds, respectively, compared with that of the wild type enzyme. The mutant retains its catalytic efficiency toward N-acetyl-D-Met compared with that of the wild type enzyme |
-, 720103 |
| 3.5.1.81 | H220A |
inactive mutant enzymes |
-, 656205 |
| 3.5.1.81 | H250N |
KM-value for N-acetyl-D-leucine is 6fold higher than the wild-type value, turnover number is 0.0246% of the wild-type value. Below 1% of the relative activity compared with wild-type enzyme.The zinc content of the mutant enzyme is 0.7 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme |
654985 |
| 3.5.1.81 | H251N |
approximately 30% of the activity of wild-type enzyme. KM-value for N-acetyl-D-leucine is 32% of the wild-type value, turnover number is 48% of the wild-type value. |
654985 |
| 3.5.1.81 | H67I |
no detectable activity |
654985 |
| 3.5.1.81 | H67N |
KM-value for N-acetyl-D-leucine is 75% of the wild-type value, turnover number is 0.0012% of the wild-type value. The zinc content of the mutant enzyme is 2.2 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme |
654985 |
| 3.5.1.81 | L298A |
site-directed mutagenesis, the catalytic efficiency of the mutant toward N-acetyl-D-Trp is enhanced by 4.4folds compared to the wild-type enzyme, with unaltered other properties, e.g. pH and temperature dependence |
-, 757308 |
