EC Number   |
Protein Variants |
Reference |
|---|
   3.4.24.63 | D240A |
site-directed mutagenesis, the D204A mutant shows almost no signal at the cell surface, but strongly colocalized with the endoplasmic reticulum marker, low cell surface activity of D204A enzyme |
733822 |
| 3.4.24.63 | D245A |
site-directed mutagenesis |
733822 |
| 3.4.24.63 | D47A |
the mutation is localized in the pro-peptide of the protease, the mutant is transported to the cell surface and exhibit expression comparable with wild-type meprin beta |
754236 |
| 3.4.24.63 | E153A |
site-directed mutagenesis, a proteolytic inactive mutant enzyme |
753536 |
| 3.4.24.63 | E90A |
soluble inactive mutant of meprin beta |
734193 |
| 3.4.24.63 | G32R |
naturally occuring mutant, the mutation is localized in the pro-peptide of the protease and identified in endometrium cancer, the mutant is transported to the cell surface and exhibit expression comparable with wild-type meprin beta, the mutant is more active against a peptide substrate (meprin beta-specific peptide substrate that is linked to a fluorogenic group (MCA) at the N-terminus and a quencher (DPN) at the C-terminus) and the interleukin-6 receptor than wild-type meprin beta. The change to an arginine residue at position 32 represents an additional activation site used by furin-like proteases in the Golgi, which consequently leads to reduced shedding by ADAM17. The meprin beta G32R variant assesses cell proliferation, invasion through a collagen IV matrix, and outgrowth from tumor spheroids. Increased meprin beta G32R activity at the cell surface reduces cell proliferation, but increases cell invasion. The G32R meprin beta variant shows increased activity. Phenotype, overview |
754236 |
| 3.4.24.63 | G32R/R61S |
site-directed mutagenesis, the double mutant has two altered sites that can no longer be proteolytically cleaved and cannot be activated or shedded |
754236 |
| 3.4.24.63 | K185Y |
site-directed mutagenesis |
652469 |
| 3.4.24.63 | more |
generation and genotyping of meprin beta-knockout mice |
-, 735308 |
| 3.4.24.63 | more |
generation of bleomycin-treated meprin alpha, meprin beta, and the double meprins alphabeta knock-out (KO) mice. No difference in lung function parameters and no change in inflammatory cells infiltrating the lung are observed between wild-type and all meprins KO mice after 14 days bleomycin. Morphological analysis in the bleomycin-treated mice reveals a decrease in collagen deposition and tissue density in meprin beta KO mice, but not in meprin alpha and meprin alphabeta KO mice. This finding is accompanied by localization of meprin beta to epithelial cells in regions with immature collagen in mice |
755466 |
