EC Number   |
Protein Variants |
Reference |
|---|
   3.4.24.36 | F448V |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
-, 717290 |
| 3.4.24.36 | F448Y |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
-, 717290 |
| 3.4.24.36 | more |
construction of several truncation mutants, deletion of about 180-211 amino acids, DELTA420 and DELTA389, from the C-terminal region results in approximately 50% reduction in residual activitycompared to the wild-type enzyme |
-, 717290 |
| 3.4.24.36 | more |
enzyme-deficient transfectants due to expression of enzyme antisense mRNA show increased generation time, altered cell morphology, accumulation of cells in G2/M phase of cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth of transfectants can be stimulated by addition of a fusion protein containing the fibronectin-like SRYD motif and the zinc-binding domain of enzyme |
669539 |
| 3.4.24.36 | more |
Leishmaina major gp63ko strain has all seven gp63 genes deleted. In general these ko parasites show a decreased virulence, but are still able to cause significant footpad swelling. Proliferation of interleukin-2-activated purified natural killer cells is suppressed after exposure to the wild-type but not to gp63ko promastigotes. gp63ko Leishmaina major induces no natural killer cell proliferation when natural killer cells are co-cultured with peripheral blood mononuclear cells populations such as CD14+ monocytes or T cells |
697408 |
| 3.4.24.36 | more |
leishmanolysin-knock out mutants show a substantially higher sensitivity to inhibitors and their antileishmanial activity, such as members of the alpha- and phi-defensins, magainins and cathelicidins, pexiganan-treatment of knock out mutants induces disruption of surface-membrane permeability and expression of features of apoptosis including smaller cell size, loss of mitochondrial membrane potential, exposure of surface phosphatidyl serine as well as induction of caspase 3/7 activity, phenotype and pexiganan cleavage products generated by leishmanolysin degradation, overview |
683897 |
| 3.4.24.36 | more |
preparation of active enzyme immobilized by glutaraldehyde |
683079 |
| 3.4.24.36 | S446A |
site-directed mutagenesis, the mutant shows 50% reduced activity and altered kinetics compared to the wild-type enzyme |
-, 717290 |
| 3.4.24.36 | S446T |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
-, 717290 |
