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EC Number
Protein Variants
Commentary
Reference
3.4.24.28
G8C/N60C
double mutant temperature optimum at 80°C, increased thermal stability
685711
3.4.24.28
G8C/N60C
double mutation
651101
3.4.24.28
G8C/N60C
introduction of disulfide bridge fixing the region near calcium binding site III. At guanidinium/HCl concentrations above 7.5 M, mutant unfolds distinctly more slowly than wild-type. Below 7.5 M guanidinium/HCl, mutant behaves similar to wild-type
668546
3.4.24.28
G8C/N60C
thermostable variant of the non-specific neutral protease
711482
3.4.24.28
I157D
site-directed mutagenesis
650655
3.4.24.28
W55F
higher Ca2+ concentration is needed than for wild-type for occupation of Ca2+-binding site III at amino acids 55-59
668546
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