EC Number   |
Protein Variants |
Reference |
|---|
   3.4.23.49 | D206A |
site-directed mutagenesis, inacticve catalytic site mutant |
-, 753675 |
| 3.4.23.49 | D208A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D208G |
site-directed mutagenesis, the mutant enzyme shows increased specificity for the A-R cleavage site compared to the wild-type enzyme |
663249 |
| 3.4.23.49 | D210A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D210A |
site-directed mutagenesis by overlap extension PCR |
753795 |
| 3.4.23.49 | D43A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D83A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D85A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D97A |
introduced as silent mutation in plasmids, transformation with plasmids |
651289 |
| 3.4.23.49 | D97A |
site-directed mutagenesis, mutant shows altered cleavage specificity compared to the wild-type enzyme, substrate specificity with fusion protein, overview |
660757 |
