EC Number |
Protein Variants |
Reference |
---|
3.4.22.70 | C184A |
catalytically inactive |
717705 |
3.4.22.70 | C184A |
mutant enzyme can not cleave the LPXTG motif |
647382 |
3.4.22.70 | C184A |
site-directed mutagenesis |
731485 |
3.4.22.70 | C184Hcy |
site-directed mutagenesis, generation of a sortase mutant with Cys184 replaced by homocysteine (Hcy). Mutant Hcy-sortase is a poor catalyst with less than 1% of wild-type activity. The sensitivity of the active site nucleophiles towards an alkylation reagent correlates with the pKa values of the mutated residues |
731485 |
3.4.22.70 | C184S |
2700fold decrease in kcat/Km compared to wild-type value |
678296 |
3.4.22.70 | C184Sec |
site-directed mutagenesis, generation of a sortase mutant with Cys184 replaced by selenocysteine (Sec). Mutant Sec-sortase shows a moderate 2-3fold reduction in catalytic activity. The sensitivity of the active site nucleophiles towards an alkylation reagent correlates with the pKa values of the mutated residues. The pH-profile of mutant Sec-sortase is shifted to more acidic conditions when compared to the wild-type enzyme |
731485 |
3.4.22.70 | D170A |
Tm is 1.7°C higher than the Tm-value of wild-type enzyme. No change in kcat/Km compared to wild-type value |
680902 |
3.4.22.70 | D170A |
turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is nearly identical to wild-type value |
665718 |
3.4.22.70 | D185A 1.3 |
fold decrease in kcat/Km compared to wild-type value |
678296 |
3.4.22.70 | D186A |
1.8fold decrease in kcat/Km compared to wild-type value |
678296 |