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Results 1 - 10 of 53 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184A catalytically inactive 717705
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184A mutant enzyme can not cleave the LPXTG motif 647382
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184A site-directed mutagenesis 731485
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184Hcy site-directed mutagenesis, generation of a sortase mutant with Cys184 replaced by homocysteine (Hcy). Mutant Hcy-sortase is a poor catalyst with less than 1% of wild-type activity. The sensitivity of the active site nucleophiles towards an alkylation reagent correlates with the pKa values of the mutated residues 731485
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184S 2700fold decrease in kcat/Km compared to wild-type value 678296
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70C184Sec site-directed mutagenesis, generation of a sortase mutant with Cys184 replaced by selenocysteine (Sec). Mutant Sec-sortase shows a moderate 2-3fold reduction in catalytic activity. The sensitivity of the active site nucleophiles towards an alkylation reagent correlates with the pKa values of the mutated residues. The pH-profile of mutant Sec-sortase is shifted to more acidic conditions when compared to the wild-type enzyme 731485
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70D170A Tm is 1.7°C higher than the Tm-value of wild-type enzyme. No change in kcat/Km compared to wild-type value 680902
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70D170A turnover-number for o-aminobenzoyl-LPETG-2,4-dinitrophenyl is nearly identical to wild-type value 665718
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70D185A 1.3 fold decrease in kcat/Km compared to wild-type value 678296
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.70D186A 1.8fold decrease in kcat/Km compared to wild-type value 678296
Results 1 - 10 of 53 > >>